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PROCEDURE
Staph_Procedure_Interpretation_1 STEP 1: Add a drop of the Latex Reagent to a well of the test card.
Staph_Procedure_Interpretation_2 STEP 2: Using a disposable stirrer, collect a visible amount of an isolated colony about 2 mm in size from the overnight culture grown on 5% sheep blood agar plate.
Staph_Procedure_Interpretation_3b
 STEP 3: Emulsify the culture sample in the Latex Reagent on the card. Discard the stirrer into an appropriate biohazard container.
Staph_Procedure_Interpretation_4 STEP 4: Gently tilt and rotate the card in a complete circular motion for up to 45 seconds, or until agglutination is evident, which ever comes first. Positive reactions usually occur within 15-20 seconds.
Staph_Procedure_Interpretation_5 STEP 5: View the mixture on the card, using only a high intensity light source. Do not use a magnifying lens.
 

INTERPRETATION

REACTIVE

 Any degree of agglutination as compared to the nonreactive control.

NONREACTIVE

 Smooth suspension with no visible agglutination after 45 seconds.
 Staph_Interpretation

STAPH Procedure