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MATERIALS PROVIDED
 
VDRL Antigen 2 x 5.0 ml
VDRL Buffered Saline 2 x 60.0 ml
 

ADDITIONAL MATERIALS REQUIRED
 

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 Mechanical rotator set at 180 ± 5 rpm and circumscribing 3/4 inch diameter, with humidity cover

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 VDRL control sera: reactive, weak reactive, nonreactive

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 Saline (0.9% NaCl Solution)

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 Non-disposable calibrated 18-gauge needle without bevel

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 Non-disposable glass syringe, 1 ml or 2 ml

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 Bottles, 30 ml, round, narrow-mouth, approximately 35 mm in diameter with ground glass stoppers and flat inner bottom surfaces

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 Slides, 2 x 3" with rings approximately 14 mm in diameter. The rings can be paraffin or ceramic, bust must be sufficiently high to prevent spillage during rotation.

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 Micropipettor, calibrated to deliver 50 µl

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 Pipets, glass serological: 1 ml in 1/10 increments, 5 ml in 1/10 increments, 10 ml in 1/10 increments

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 Timing device, minute and second capability

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 Microscope capable of 100x magnification
 
 

 
 
 
 
 
 
 
 

VDRL Materials Provided