Using a fresh stirrer pipet each time, add one drop (0.05ml) of each sample onto a separate circle on the test card. Repeat by adding one drop of REACTIVE or NONREACTIVE CONTROL from the dropper vials supplied.
Shake the vial of Color Mono Latex Reagent gently to uniformly mix the suspension. Add one free-falling drop of reagent to each control and sample.
Using the flat end of the stirrer pipets, mix each sample and reagent and spread over the entire cirlce.
Gently rotate the card for 2 minutes. Observe for agglutination under high intensity light. All test results should be compared to both REACTIVE and NONREACTIVE CONTROLS.
Reactive: Any degree of agglutination or rimming within the test area as compared to the nonreactive control.
Nonreactive: Smooth or finely granular suspension with no visible agglutination.
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