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Color Mono II Test Procedures

Using a fresh stirrer pipet each time, add one drop (0.05ml) of each sample onto a separate circle on the test card.
Shake the vial of latex reagent gently to uniformly mix the suspension. Add one free-falling drop of reagent to each sample.
Using the flat end of the stirrer pipets, mix each sample and reagent and spread over the entire cirlce. Use a new pipet for each sample
Gently hand rotate card for two minutes. Read results under a bright light.

Step 1:

Using a fresh stirrer pipet each time, add one drop (0.05ml) of each sample onto a separate circle on the test card. Repeat by adding one drop of REACTIVE or NONREACTIVE CONTROL from the dropper vials supplied.

 

 

Step 2:

Shake the vial of Color Mono Latex Reagent gently to uniformly mix the suspension. Add one free-falling drop of reagent to each control and sample.

 

 

Step 3:

Using the flat end of the stirrer pipets, mix each sample and reagent and spread over the entire cirlce.

 

 

Step 4:

Gently rotate the card for 2 minutes. Observe for agglutination under high intensity light. All test results should be compared to both REACTIVE and NONREACTIVE CONTROLS.

 

Interpretation

Reactive: Any degree of agglutination or rimming within the test area as compared to the nonreactive control.

Nonreactive: Smooth or finely granular suspension with no visible agglutination.

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ADDITIONAL INFORMATION

Reactive shows any degree of agglutination or rimming in test area. Nonreactive will be smooth or finely granular with no visible agglutination.

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Rev. 01_2019

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