Using a fresh stirrer pipet each time, add a drop (0.05) ml of each sample onto a separate circle on the test card. Repeat by adding one drop of REACTIVE or NONREACTIVE CONTROL from the dropper vials supplied.
Expel the contents of the LATEX REAGENT dropper and refill. Add one drop of the reagent to each serum specimen and to each control.
Using the flat end of the stirrer pipets, mix each sample and control serum with the LATEX REAGENT, in a circular manner, over the entire area in the circles of the card.
Gently tilt and rotate the card for two (2) minutes and observe for agglutination. All test results should be compared to both REACTIVE and NON REACTIVE CONTROLS.
Reactive: Agglutination indicates an RF concentration of greater than or equal to 8 IU/ml in the serum sample.
Nonreactive: Smooth or finely granular suspension with no visible agglutination.
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