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Repeat by adding one drop of reactive or nonreactive control from the dropper vials supplied.

1

Add one drop of the reagent to each serum specimen and to each control.

2

Mix sample and control serum with latex reagent in a circular motion and cover entire area of test circle.

3

After rotating card for two minutes, observe samples for agglutination and compare to reactive and non-reactive controls.

4

RF Procedure and Interpretation

Step 1:

Using a fresh stirrer pipet each time, add a drop (0.05) ml of each sample onto a separate circle on the test card. Repeat by adding one drop of REACTIVE or NONREACTIVE CONTROL from the dropper vials supplied.

Step 2:

Expel the contents of the LATEX REAGENT dropper and refill. Add one drop of the reagent to each serum specimen and to each control.

Step 3:

Using the flat end of the stirrer pipets, mix each sample and control serum with the LATEX REAGENT, in a circular manner, over the entire area in the circles of the card.

Step 4:

Gently tilt and rotate the card for two (2) minutes and observe for agglutination. All test results should be compared to both REACTIVE and NON REACTIVE CONTROLS.

Interpretation

Reactive: Agglutination indicates an RF concentration of greater than or equal to 8 IU/ml in the serum sample.

Nonreactive: Smooth or finely granular suspension with no visible agglutination.

Test shows a reactive and nonreactive RF test result for purposes of interpreting the test.

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Rev. 01_2019

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