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Rubella Test Procedure & Interpretation

Place 25μl of the sample onto one of the circles of the disposable card.
Using a new plastic stirrer for each circle, spread the sample over the entire surface of the card.
Dispense one drop of the latex reagent onto each circle containing the sample.
Place the card on an automatic rotator and cover to maintain humidity. Rotate at 100±5 rpm for 8 minutes.
Immediately following the 8 minute rotation, read for the presense or absence of agglutination.

Step 1:

Place 25μl of the sample onto one of the circles of the disposable card.

 

 

 

Step 2:

Using a new plastic stirrer for each circle, spread the sample over the entire surface of the card.

 

 

 

Step 3:

Dispense one drop of the latex reagent onto each circle containing the sample.

 

 

Step 4:

Place the card on an automatic rotator and cover to maintain humidity. Rotate at 100±5 rpm for 8 minutes.

 

 

 

Step 5:

Immediately following the 8 minute rotation, read for the presense or absence of agglutination.

Interpretation

Reactive: The presence of any visible agglutination, significantly different from the nonreactive control, indicates the presence of antibodies against rubella virus in the serum sample.

Nonreactive: Smooth or finely granular suspension with no visible agglutination. When a nonreactive result is obtained on undiluted serum, the sample should be retested at 1:10, as occasionally a decrease in the degree of agglutination has been reported with high titered specimens.

ADDITIONAL INFORMATION

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Reactive shows the presence of any visible agglutination. Nonreactive shows a finely granular suspension with no visible agglutination.

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