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Preparation of working sickle cell buffer
Add the entire contents of one vial of Sickle Cell Lysing Reagent to 50ml of Sickle Cell Buffer and mix completely by swirling. The working buffer may be used for 30 days from date of preparation if stored at 2° to 8° C.
Reactive - If any sickling hemoglobin is present, the solution will be sufficiently turbid to prevent reading the Line Scale through the test tube.
Nonreactive - If a sickling hemoglobin is not present, the solution will be clear enough to allow the Line Scale to be seen through the test tube.
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