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Staph Procedures and Interpretation

Add a drop of the latex reagent to a well of the test card.
Use a disposable stirrer to collect a visible amount of an isolated colony about 2mm from a culture grown on 5% sheep blood agar plate.
Emulsify the culture sample in the latex reagent on the card. Discard the stirrer in an appropriate biohazard container
Tilte and rotate the card in a circular motion for 45 seconds or until agglutination is evident. Reactions occure within 15 to 20 seconds.
View the mixture on the card using a bright light source. Do not use a magnifying lens.

Step 1

Add a drop of the Latex Reagent to a well of the test card.

 

 

 

Step 2

Using a disposable stirrer, collect a visible amount of an isolated colony about 2 mm in size from the overnight culture grown on 5% sheep blood agar plate.

 

 

 

Step 3

Emulsify the culture sample in the Latex Reagent on the card. Discard the stirrer into an appropriate biohazard container.

 

 

 

Step 4

Gently tilt and rotate the card in a complete circular motion for up to 45 seconds, or until agglutination is evident, which ever comes first. Positive reactions usually occur within 15-20 seconds.

 

 

Step 5

View the mixture on the card, using only a high intensity light source. Do not use a magnifying lens

 

Interpretation

Reactive - Any degree of agglutination as compared to the nonreactive control.

Nonreactive - Smooth suspension with no visible agglutination after 45 seconds.

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ADDITIONAL INFORMATION

Reactive is any degree of agglutination. Nonreactive shows a smooth suspension with no visible agglutination after 45 seconds.

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